Thursday 29 December 2016
Thursday 22 December 2016
Thursday 15 December 2016
Friday 9 December 2016
Thursday 1 December 2016
Thursday 24 November 2016
Thursday 10 November 2016
Call For Paper
Bentham Science Publishers would like to invite you to submit your research paper for publishing in the Journal of
Thursday 3 November 2016
Highlighted Artcile: Identification and Characterization of Amniotic Fluid Proteins Incident to Normal, Preeclampsia and Polyhydramnios Pregnancies
Identification and Characterization of Amniotic Fluid Proteins Incident to Normal, Preeclampsia and Polyhydramnios Pregnancies
Author(s):
Ruta Navakauskiene, Sandra Baronaite, Dalius Matuzevicius, Ilona Zaikova, Audrone Arlauskiene, Dalius Navakauskas and Grazina Treigyte Pages 206 - 217 ( 12 )
Abstract:
Background: Amniotic fluid is a complex mixture and reflects the physiological status of the developing fetus. Many proteins presented in amniotic fluid are of exceptional interest because their expression reflects physiological and pathological conditions of the fetus and/or pregnancy.
Objective: In the present study, we performed proteomics-based identification of biomarkers of amniotic fluid from normal and pathological pregnancy.
Methods: Proteins isolated from amniotic fluid of three different status of pregnancy - normal, preeclampsia and polyhydramnios, were fractionated by 2DE, visualized by Coomassie blue staining and all visualized proteins subjected for identification with mass spectrometry. Around 40 proteins were identified by mass spectrometry analysis. Changes in identified protein levels defined in three different pregnancy status were evaluated by using computational methods.
Results: In our study we characterized proteins isolated from amniotic fluid obtained from normal pregnancy, developed preeclampsia and polyhydramnios. Some proteins’ are variable in their expression level in comparison to pathological cases with normal pregnancy. These proteins are involved in various cellular processes and are responsible for cell signaling and regulation (apolipoprotein, peroxiredoxin, protein AMBP, putative elongation factor, clusterin, etc.), metabolic processes (lumican, vitamin D binding protein, etc.), macromolecular transport (ceruloplasmin, corticosteroidbinding protein, etc.), proteins specific for pregnancy and embryo development (angiotensinogen, fibrinogen beta chain, etc.) and others.
Conclusion: In the study new proteins typical for amniotic fluid of preeclampsia and polyhydramnios pregnancies were identified. These proteins are associated with development, signal transduction, and metabolic processes. Results could be valuable for developing biomarkers for fetal development and pregnancy-related disorders.
Keywords:
Amniotic fluid, preeclampsia, polyhydramnios, pregnancy, proteomics.
Affiliation:
Department of Molecular Cell Biology, Institute of Biochemistry, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, Lithuania.
Graphical Abstract:
For More Information Please Visit Our Website Current Proteomics
Wednesday 26 October 2016
Most Accessed Article: Use of Filter-Aided Capture and Elution Protocol for Concurrent Preparation of N-glycan and O-glycopeptides for LC-MS/MS Analysis
Use of Filter-Aided
Capture and Elution Protocol for Concurrent Preparation of N-glycan and
O-glycopeptides for LC-MS/MS Analysis
Author(s):
Albert-Baskar Arul, Jong-Moon Park, Hookeun
Lee, Na-Young Han and Jongho JeonPages 48-54 (7)
Abstract:
Glycosylation, one of the most common types of post-translational modification (PTM), is frequently observed in membrane and plasma proteins. Characterization of glycan structures (participating in glycosylation) using mass spectrometry is important in biopharmaceutical industry. The present study describes a novel and improved N-glycan enrichment method using a filter aided capture and elution protocol. The glycopeptides of human IgG digests were selectively captured by binding to lectins, and the remaining non-glycopeptides were washed off by allowing them to pass through the membrane. The lectin binding glycopeptides were treated with Peptide -N-Glycosidase F (PNGase F), which cleaves the bond between arginine and glycan, and the de-glycosylated peptides were selectively obtained by filter-aided capture and elution method. After eluting the de-glycosylated peptides, the N-glycans and O-glycopeptides attached to the lectins were released by washing with 80% acetonitrile. The eluted N-glycan moieties and the intact O-glycopeptides were directly injected to the LC-MS/MS system without further enrichment. We identified 22 N-glycan moieties from a single standard human IgG protein. This novel protocol allows the enrichment and elution of N-Glycan and intact O-glycopeptides from a single experimental batch.
Keywords:
Fetuin, filter aided capture and elution,
human IgG, LC-MS/MS, lectin enrichment, N-Glycan analysis, Oglycopeptide.
Affiliation:
College of Pharmacy, Gachon University, 7-45
Songdo, Yeonsu, Incheon 406 799, Korea.
Graphical Abstract:
Thursday 20 October 2016
Wednesday 5 October 2016
High Impact Factor Journal ::: Current Proteomics
Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.
Abstracted & Indexed in:
Science Citation Index ExpandedTM, Journal Citation Reports/Science Edition, Journal Citation Reports®, Index to Scientific Reviews®, Biochemistry & Biophysics Citation Index®, BIOSIS, BIOSIS Previews, BIOSIS Reviews Reports and Meetings, MEDLINE/PubMed/Index Medicus, Scopus, EMBASE/Excerpta Medica, Chemical Abstracts, ProQuest, BIOBASE, EMBiology, PubsHub, Genamics JournalSeek, MediaFinder®-Standard Periodical Directory and J-Gate.
courtesy by https://benthamsciencepublishers.wordpress.com/2015/09/30/high-impact-factor-journal-current-proteomics/
Recently Published Issue of the Journal Current Proteomics
Current Proteomics publishes review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, softwares, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to:
| Protein separation and characterization techniques
2-D gel electrophoresis and image analysis
Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching
Determination of co-translational and post- translational modification of proteins
Protein/peptide microarrays
Biomolecular interaction analysis
Analysis of protein complexes
Yeast two-hybrid projects
| Protein-protein interaction (protein interactome) pathways and cell signaling networks
Systems biology
Proteome informatics (bioinformatics)
Knowledge integration and management tools
High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography)
High-throughput computational methods for protein 3-D structure as well as function determination
Robotics, nanotechnology, and microfluidics
|
In addition to in-depth reviews, articles highlighting recent proteomics papers of special interest as well as book reviews are published. Mini-reviews and perspective articles are also published. In view of the importance of specific technology and software developed for advancement of proteomics research, a separate section is devoted to product and technology reports. Proceedings of proteomics meetings with selected review articles on topics presented are also published.
Following are the articles from the journal Current Proteomics, 11 Issue 3:
Article: B-factor Analysis and Conformational Rearrangement of Aldose Reductase
Author(s): Ganesaratnam K. Balendiran, J. Rajendran Pandian, Evin Drake, Anubhav Vinayak, Malkhey Verma and Duilio Cascio
Article: Plant Protein Extraction and Identification from Eurycoma longifolia by Gel Electrophoresis and Mass Spectrometry
Author(s): Lee Suan Chua, Nurulaini Abd Rahman and Mohamad Roji Sarmidi
Article: Interactions Between Apo E and Amyloid Beta and their Relationship to Nutriproteomics and Neurodegeneration
Author(s): Ian J. Martins, Veer Gupta, Andrea C. Wilson, Stephanie J. Fuller and Ralph N. Martins
Article: Effective Inhibition of Foam Cells Formation by Tanshinone IIA in RAW264.7 Macrophages Induced with LDL Isolated from Hypercholesterolemia Patients: A Proteomic Analysis
Author(s): De-zhao Lu, Yun Zhou, Ji Zhu, Yao-hong Teng, Zhen Yang, Hui Chai and Li-ke Wo
Article: Proteomics and Epigenetic Mechanisms in Stem Cells
Author(s): Sabata Martino, Francesco Morena, Carolina Barola, Ilaria Bicchi and Carla Emiliani
Article: Oncogenic Signalling Networks and Polypharmacology as Paradigms to Cope with Cancer Heterogeneity
Author(s): Aurelio A. Moya GarcÃa, Ian Morilla and Juan Juan A.G. Ranea
Article: Proteomic Differential in-Gel Electrophoresis Analysis of Stigmatic and Stylar Proteins in Kiwifruit before and after Pollination
Author(s): Xiujuan Qi, Yongbin Gao, Jinbao Fang, Shankun Xu, Jinyong Chen, Hong Gu and Shaoling Zhang
For details, please visit: http://bit.ly/1LKipIT
courtesy by https://benthamsciencepublishers.wordpress.com/2015/03/13/recently-published-issue-of-the-journal-current-proteomics-2/
BSP Journals Impacting Science: ‘Current Pharmaceutical Analysis’ and ‘Current Proteomics’
Current Pharmaceutical Analysis
Editor-in-Chief:
Atta-ur-Rahman, FRS
Honorary Life Fellow
Kings College
University of Cambridge
Cambridge
UK
Kings College
University of Cambridge
Cambridge
UK
Aims & Scope
Current Pharmaceutical Analysis publishes expert reviews and original research articles on all the most recent advances in pharmaceutical and biomedical analysis. All aspects of the field are represented including drug analysis, analytical methodology and instrumentation. The journal is essential to all involved in pharmaceutical, biochemical and clinical analysis.
Current Proteomics
Editor-in-Chief:
Bernd Rehm
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand
Private Bag 11222
Palmerston North
New Zealand
Aims & Scope
Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.
Current Proteomics publishes review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, softwares, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to:
|
|
In addition to in-depth reviews, articles highlighting recent proteomics papers of special interest as well as book reviews are published. Mini-reviews and perspective articles are also published. In view of the importance of specific technology and software developed for advancement of proteomics research, a separate section is devoted to product and technology reports. Proceedings of proteomics meetings with selected review articles on topics presented are also published.
courtesy by https://benthamsciencepublishers.wordpress.com/2014/06/26/bsp-journals-impacting-science-current-pharmaceutical-analysis-and-current-proteomics/
Highlighted Article Flyer for the journal “Current Proteomics” Volume 13, Number 2, 2016
courtesy by https://benthamsciencepublishers.wordpress.com/2016/08/30/highlighted-article-flyer-for-the-journal-current-proteomics/
Friday 12 August 2016
Protein Biomarkers in Cancers of the Digestive Tract - a Step Towards Personalized Medicine
Author(s):
Erno Duda, Elena Codrici, Daniela Ionela Popescu, Laura Necula and Radu AlbulescuPages 228-236 (9)
Abstract:
Digestive tract cancers – gastric-, colorectal-, pancreatic-, hepatocarcinoma- and esophageal are some of the most frequent cancers; they are characterized by invasivity, metastatic potential and bad outcomes. This group includes several of the most critical cancers (those ranked 2nd-4th in cancer related mortality), and, despite all efforts, they remain with low survival rates and lack of success of therapies. Discovery of novel biomarkers may improve disease characterization and make optimized or personalized therapies possible. The novel biomarkers are expected to provide, hopefully, less invasive or non-invasive diagnostic tools to make possible earlier detection of disease, and, also, they will provide more reliable selection in the drug discovery process, and provide guidance for personalized medicine.
Deregulation of protein expression and genetic alterations were demonstrated in various cancers, including digestive. Investigations in tissue samples provided a considerable amount of knowledge, identifying altered expressions of proteins associated with tumorigenesis and tumour progression. Over-expression of some tumour-inducing or tumour promoting proteins was demonstrated, as well as expression down-regulation of several tumor suppressor genes. Often mutated and polymorphic alleles were demonstrated to occur in various cancers with high incidence. Several protein biomarkers were also demonstrated to be differentially expressed in groups of patients showing different responsivities to therapies.
Both individual proteins and sets of multiple proteins were set up as candidate biomarkers for diagnostics or monitoring, offered relevant insights on the tumorigenic mechanisms. Proteins and other molecules (mRNAs, miRNAs, lncRNAs) are also providing potential candidates for multifactorial panels of biomarkers.
Keywords:
Biomarkers, colorectal cancer, gastric cancer, hepatocellular carcinoma, pancreatic cancer, protein biomarker.
Affiliation:
Institute of Pathology, 99-101 Spl. Independentei, Bucharest, Romania.
Read Full-Text article
Anti-cancer Therapies in High Grade Gliomas
Author(s):
Cristiana Pistol Tanase, Ana-Maria Enciu, Simona Mihai, Ana Iulia Neagu, Bogdan Calenic and Maria Linda CruceruPages 246-260 (15)
Abstract:
High grade gliomas represent one of the most aggressive and treatment-resistant types of human cancer, with only 1–2 years median survival rate for patients with grade IV glioma. The treatment of glioblastoma is a considerable therapeutic challenge; combination therapy targeting multiple pathways is becoming a fast growing area of research. This review offers an up-to-date perspective of the literature about current molecular therapy targets in high grade glioma, that include angiogenic signals, tyrosine kinase receptors, nodal signaling proteins and cancer stem cells related approaches. Simultaneous identification of proteomic signatures could provide biomarker panels for diagnostic and personalized treatment of different subsets of glioblastoma. Personalized medicine is starting to gain importance in clinical care, already having recorded a series of successes in several types of cancer; nonetheless, in brain tumors it is still at an early stage.
Keywords:
Antiangiogenic therapy, cancer stem cells, glioma, microRNA, personalized medicine, PI-3K.
Affiliation:
Victor Babes National Institute of Pathology, Dept. of Biochemistry-Proteomics, no 99-101 Splaiul Independentei, 050096 sect 5 Bucharest, Romania.
Read Full-Text article
A Simple Protein Extraction Method for Proteomic Analysis of Diverse Biological Specimens
Author(s):
Panga Jaipal Reddy, Aishwarya Anand Rao, Darpan Malhotra, Samridhi Sharma, Ravinder Kumar, Rekha Jain, Kishore Gollapalli, Namita Pendharkar, Srikanth Rapole and Sanjeeva SrivastavaPages 298-311 (14)
Abstract:
The success of a proteomic experiment largely depends on the quality and quantity of the protein extract. Currently, various protocols are available for extraction of proteins from different types of samples; however, further optimization is required for every new sample type. Hence, a common protein extraction protocol is desirable. In the present study, soluble proteins were extracted from six diverse samples using TRIzol without any additional clean-up step and subjected to 2-DE and 2D-DIGE analysis for global protein expression profiling. Image analysis using IMP7 and DeCyder showed good coverage, reproducibility and quality of the gel. MS analysis of 24 spots from all the six samples showed good score and coverage for the identified proteins. Additionally, this method facilitated the concurrent isolation of RNA from the same cell lysates with high integrity and quality, suitable for transcriptomic analysis. Thus, we demonstrate the use of a common protein extraction protocol involving TRIzol reagent for 2-DE, 2D-DIGE and MS analysis using six diverse samples and show its suitability for concomitant transcriptomic studies.
Keywords:
2-DE, 2D-DIGE, breast cancer, B. subtilis, glioblastoma cell line, mass spectrometry, protein extraction, S. coelicolor, TRIzol, yeast.
Affiliation:
Department of Biosciences and Bioengineering, IIT Bombay, Mumbai 400 076, India.
Read Full-Text article
Important Anti-Cancer Applications of Protein Based Nanoparticles
Author(s):
Mustafa Ergul, Merve Ergul and Yusuf TutarPages 334-340 (7)
Abstract:
Cancer is one of the most important health problems in the world and the treatment approaches are still challenging and generally not at desirable levels. Conventional anti-cancer agents may cause systemic toxicity, have poor solubility, and lack of selectivity. In order to overcome these hurdles, nanoparticle based therapeutics have been widely employed to enhance therapeutic efficacies and reduce systemic toxicity of pharmaceutical agents. In recent years, nanoparticulate carrier systems (NPCs) in medicine have attracted considerable scientific attention and interest in the worldwide because of their unique properties in the diagnosis and therapy of many diseases including cancer. One of the therapeutic strategies of NPCs is protein based nanoparticles. Human serum albumin (HSA) and bovine serum albumin (BSA) are the most frequently used materials for this purpose due to their remarkable advantages. In this review article, we focused on protein based nanoparticles and summarized their important applications including anti-cancer drug delivery, photodynamic therapy, and gene delivery.
Keywords:
Anti-cancer drug delivery, bovine serum albumin, gene delivery, human serum albumin, photodynamic therapy, protein based nanoparticles.
Affiliation:
Division of Biochemistry, Cumhuriyet University, 58140, Sivas, Turkey.
Read Full-Text article
B-factor Analysis and Conformational Rearrangement of Aldose Reductase
Author(s):
Ganesaratnam K. Balendiran, J. Rajendran Pandian, Evin Drake, Anubhav Vinayak, Malkhey Verma and Duilio CascioPages 151-160 (10)
Abstract:
The NADPH-dependent reduction of glucose reaction that is catalyzed by Aldose Reductase (AR) follows a sequential ordered kinetic mechanism in which the co-factor NADPH binds to the enzyme prior to the aldehyde substrate. The kinetic/structural experiments have found a conformational change involving a hinge-like movement of a surface loop (residues 213-224) which is anticipated to take place upon the binding of the diphosphate moiety of NADPH. The reorientation of this loop, expected to permit the release of NADP+, represents the rate-limiting step of the catalytic mechanism. This study reveals: 1) The Translation/Libration/Screw (TLS) analysis of absolute B-factors of apo AR crystal structures indicates that the 212-224 loop might move as a rigid group. 2) Residues that make the flexible loop slide in the AR binary and ternary complexes. 3) The normalized B-factors separate this segment into three differnt clusters with fewer residues.
Keywords:
Aldo-keto reductase, B-factor, clustering, crystal structure, statistical analysis, structural dynamics, TLS.
Affiliation:
Department of Chemistry, WBSH 6017, Youngstown State University, One University Plaza, Youngstown, OH 44555, USA.
Graphical Abstract:
Read Full-Text article
