Wednesday 26 October 2016

Most Accessed Article: Use of Filter-Aided Capture and Elution Protocol for Concurrent Preparation of N-glycan and O-glycopeptides for LC-MS/MS Analysis



Use of Filter-Aided Capture and Elution Protocol for Concurrent Preparation of N-glycan and O-glycopeptides for LC-MS/MS Analysis
Author(s):
Albert-Baskar Arul, Jong-Moon Park, Hookeun Lee, Na-Young Han and Jongho JeonPages 48-54 (7)
Abstract:

Glycosylation, one of the most common types of post-translational modification (PTM), is frequently observed in membrane and plasma proteins. Characterization of glycan structures (participating in glycosylation) using mass spectrometry is important in biopharmaceutical industry. The present study describes a novel and improved N-glycan enrichment method using a filter aided capture and elution protocol. The glycopeptides of human IgG digests were selectively captured by binding to lectins, and the remaining non-glycopeptides were washed off by allowing them to pass through the membrane. The lectin binding glycopeptides were treated with Peptide -N-Glycosidase F (PNGase F), which cleaves the bond between arginine and glycan, and the de-glycosylated peptides were selectively obtained by filter-aided capture and elution method. After eluting the de-glycosylated peptides, the N-glycans and O-glycopeptides attached to the lectins were released by washing with 80% acetonitrile. The eluted N-glycan moieties and the intact O-glycopeptides were directly injected to the LC-MS/MS system without further enrichment. We identified 22 N-glycan moieties from a single standard human IgG protein. This novel protocol allows the enrichment and elution of N-Glycan and intact O-glycopeptides from a single experimental batch.
Keywords:
Fetuin, filter aided capture and elution, human IgG, LC-MS/MS, lectin enrichment, N-Glycan analysis, Oglycopeptide.
Affiliation:
College of Pharmacy, Gachon University, 7-45 Songdo, Yeonsu, Incheon 406 799, Korea.
Graphical Abstract:





For More Information Please Visit Our Website Current Proteomics


Thursday 20 October 2016

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Wednesday 5 October 2016

Podcast “Heat Shock Protein 90 Inhibitors in Oncology” by Dr. Yusaf Tutar

Podcast “Heat Shock Protein 90 Inhibitors in Oncology” by Dr. Yusaf Tutar


High Impact Factor Journal ::: Current Proteomics



Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.
Abstracted & Indexed in:
Science Citation Index ExpandedTM, Journal Citation Reports/Science Edition, Journal Citation Reports®, Index to Scientific Reviews®, Biochemistry & Biophysics Citation Index®, BIOSIS, BIOSIS Previews, BIOSIS Reviews Reports and Meetings, MEDLINE/PubMed/Index Medicus, Scopus, EMBASE/Excerpta Medica, Chemical Abstracts, ProQuest, BIOBASE, EMBiology, PubsHub, Genamics JournalSeek, MediaFinder®-Standard Periodical Directory and J-Gate.
cp-flyer

mostopen ccesstrial request2015-08-24_10-53-37

 

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Recently Published Issue of the Journal Current Proteomics





Current Proteomics publishes review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, softwares, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to:
Protein separation and characterization techniques
2-D gel electrophoresis and image analysis
Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching
Determination of co-translational and post- translational modification of proteins
Protein/peptide microarrays
Biomolecular interaction analysis
Analysis of protein complexes
Yeast two-hybrid projects
Protein-protein interaction (protein interactome) pathways and cell signaling networks
Systems biology
Proteome informatics (bioinformatics)
Knowledge integration and management tools
High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography)
High-throughput computational methods for protein 3-D structure as well as function determination
Robotics, nanotechnology, and microfluidics
In addition to in-depth reviews, articles highlighting recent proteomics papers of special interest as well as book reviews are published. Mini-reviews and perspective articles are also published. In view of the importance of specific technology and software developed for advancement of proteomics research, a separate section is devoted to product and technology reports. Proceedings of proteomics meetings with selected review articles on topics presented are also published.
Following are the articles from the journal Current Proteomics, 11 Issue 3:
Article: B-factor Analysis and Conformational Rearrangement of Aldose Reductase
Author(s): Ganesaratnam K. Balendiran, J. Rajendran Pandian, Evin Drake, Anubhav Vinayak, Malkhey Verma and Duilio Cascio

Article: Plant Protein Extraction and Identification from Eurycoma longifolia by Gel Electrophoresis and Mass Spectrometry
Author(s): Lee Suan Chua, Nurulaini Abd Rahman and Mohamad Roji Sarmidi

Article: Interactions Between Apo E and Amyloid Beta and their Relationship to Nutriproteomics and Neurodegeneration
Author(s): Ian J. Martins, Veer Gupta, Andrea C. Wilson, Stephanie J. Fuller and Ralph N. Martins

Article: Effective Inhibition of Foam Cells Formation by Tanshinone IIA in RAW264.7 Macrophages Induced with LDL Isolated from Hypercholesterolemia Patients: A Proteomic Analysis
Author(s): De-zhao Lu, Yun Zhou, Ji Zhu, Yao-hong Teng, Zhen Yang, Hui Chai and Li-ke Wo

Article: Proteomics and Epigenetic Mechanisms in Stem Cells
Author(s): Sabata Martino, Francesco Morena, Carolina Barola, Ilaria Bicchi and Carla Emiliani

Article: Oncogenic Signalling Networks and Polypharmacology as Paradigms to Cope with Cancer Heterogeneity
Author(s): Aurelio A. Moya GarcĂ­a, Ian Morilla and Juan Juan A.G. Ranea

Article: Proteomic Differential in-Gel Electrophoresis Analysis of Stigmatic and Stylar Proteins in Kiwifruit before and after Pollination
Author(s): Xiujuan Qi, Yongbin Gao, Jinbao Fang, Shankun Xu, Jinyong Chen, Hong Gu and Shaoling Zhang

For details, please visit: http://bit.ly/1LKipIT
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BSP Journals Impacting Science: ‘Current Pharmaceutical Analysis’ and ‘Current Proteomics’



Current Pharmaceutical Analysis

Editor-in-Chief:
Atta-ur-Rahman, FRS
Honorary Life Fellow
Kings College
University of Cambridge
Cambridge
UK

Aims & Scope

Current Pharmaceutical Analysis publishes expert reviews and original research articles on all the most recent advances in pharmaceutical and biomedical analysis. All aspects of the field are represented including drug analysis, analytical methodology and instrumentation. The journal is essential to all involved in pharmaceutical, biochemical and clinical analysis.

Current Proteomics

Editor-in-Chief:
Bernd Rehm
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand

Aims & Scope

Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.
Current Proteomics publishes review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, softwares, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to:
  • Protein separation and characterization techniques
  • 2-D gel electrophoresis and image analysis
  • Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching
  • Determination of co-translational and post- translational modification of proteins
  • Protein/peptide microarrays
  • Biomolecular interaction analysis
  • Analysis of protein complexes
  • Yeast two-hybrid projects
  • Protein-protein interaction (protein interactome) pathways and cell signaling networks
  • Systems biology
  • Proteome informatics (bioinformatics)
  • Knowledge integration and management tools
  • High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography)
  • High-throughput computational methods for protein 3-D structure as well as function determination
  • Robotics, nanotechnology, and microfluidics

In addition to in-depth reviews, articles highlighting recent proteomics papers of special interest as well as book reviews are published. Mini-reviews and perspective articles are also published. In view of the importance of specific technology and software developed for advancement of proteomics research, a separate section is devoted to product and technology reports. Proceedings of proteomics meetings with selected review articles on topics presented are also published.
courtesy by https://benthamsciencepublishers.wordpress.com/2014/06/26/bsp-journals-impacting-science-current-pharmaceutical-analysis-and-current-proteomics/

Highlighted Article Flyer for the journal “Current Proteomics” Volume 13, Number 2, 2016



CP-Articles_13-2- Jun-Wang
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